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- 横濱 道成
- 競走馬理化学研究所
書誌事項
- タイトル別名
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- Functional Heterogeneity of Equine Transferrin by Two-dimensional Electrophoresis
- 2ジゲン デンドウ エイドウホウ ニ ヨル バ Transferrin ノ f
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抄録
Heterogeneity of equine transferrin (Tf) protein was analyzed about 15 Tf phenotypes composing of D, F, H, O and R alleles by means of two-dimensional electrophoresis (2-D method).And five samples per each Tf type were used.Each heterogeneity of the Tf O and R types having slow running mobility was detected as eight spots (T1-T4 and T′1…T′4), because main-bands (T) and sub-bands (T′) of both Tf types were separated and had four spots with different pI value, respectively.However, each heterogeneity of Tf D, F and H types with fast running mobility was observed as four spots (1-4), because their T and T′ components were mutually overlapped due to the narrow mobility between both components.Although heterogeneity patterns within each Tf type were bassically same ones, some changes were observed in the number and density of spots.T and T′ components of the Tf types were changed respectively from four to one spot by treating the serum with1% ammonium ferrous sulfate solution (1: 10).From these facts, it was assumed that the heterogeneity of equine Tf protein by the 2-D method is a functional one which changes by binding with iron ion and so on.
収録刊行物
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- 日本畜産学会報
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日本畜産学会報 58 (5), 403-406, 1987
公益社団法人 日本畜産学会
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詳細情報 詳細情報について
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- CRID
- 1390001205192808576
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- NII論文ID
- 130000739522
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- NII書誌ID
- AN00195188
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- ISSN
- 18808255
- 00215309
- 1346907X
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- NDL書誌ID
- 3135442
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
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- 使用不可