ラットに混餌投与されたカラギナンの糞中への排泄量と分子量

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  • Average Molecular Weights and the Contents of Fecal Carrageenan in Rats Orally Given Carrageenan Measured by GPC/ICP-AES Method
  • ラット ニ コンジ トウヨ サレタ カラギナン ノ フン チュウ エ ノ ハイセツリョウ ト ブンシリョウ

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Carrageenan is one of a few high-molecular weight substances, which have a lot of sulfur atoms in their molecules. It may be possible to measure easily average molecular weight and content of carrageenan in vivo by GPC/ICP-AES method, which can detect sulfur selectively in high-molecular weight substances. In this paper, we described the average molecular weights and the contents of fecal carrageenan in rats orally given λ-type refined carrageenan measured by the method. Seven-week-old, Sprague-Dawley rats (one male and one female) were given the mixed diet powder containing 5% of λ-type refined carrageenan for one day. After that, they were given the basic diet powder without carrageenan for 2 days. The feces of rats were collected at the points of 1, 2 and 3 days after the beginning of the mixed diet administration, and stored at -20℃. The consumption of carrageenan sample was 1.2 g/day in male, and 0.91 g/day in female. The number average molecular weight (Mn) of carrageenan sample in feces collected at 1 day after administration was 355 kDa, and the weight average molecular weight (Mw) was 782 kDa in average of two rats. The Mn of fecal carrageenan sample collected at 2 days after admimistration was 303 kDa and the Mw was 718 kDa. It was considered that almost all of λ-type refined carrgeenan fed rats was not degraded via the gut, because the Mn of carrageenan sample in the mixed diet was 338 kDa and the Mw was 832 kDa, measured by GPC/ICP-AES method. The amount of carrageenan sample in feces collected at 1 day after administration was 659 mg in male and 466 mg in female, at 2 days after administration, 377 mg in male and 329 mg in female, and at 3 days after, 10 mg and 6.3 mg, respectively. Therefore, it was considered that almost all of carrageenan sample taken by rats was excreted in feces within 1 day, regarding the schedule of administrations and fecal collections. The recovery of fecal carrageenan sample against the taken was about 90%. It was considered that the recovery of carrageenan sample from feces might depend on the extraction ratio of carrageenan from feces, and the extraction ratio of carrageenan sample would be a further subject.

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