Detection of Recombinant DNA in Genetically Modified Soybeans and Tofu
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- Yamaguchi Hideaki
- Graduate School of Environmental Sciences, Aomori University
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- Sasaki Kazuo
- Department of Bioscience and Biotechnology, Faculty of Engineering, Aomori University
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- Kidachi Yumi
- Department of Bioscience and Biotechnology, Faculty of Engineering, Aomori University
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- Shirama Kazushi
- Department of Bioscience and Biotechnology, Faculty of Engineering, Aomori University
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- Kiyokawa Shigeto
- Department of Bioscience and Biotechnology, Faculty of Engineering, Aomori University
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- Ryoyama Kazuo
- Department of Bioscience and Biotechnology, Faculty of Engineering, Aomori University
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- Matsuoka Takeshi
- National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries
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- Hino Akihiro
- National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries
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- Umetsu Hironori
- Department of Bioscience and Biotechnology, Faculty of Engineering, Aomori University
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- Kamada Hiroshi
- Institute of Biological Sciences, University of Tsukuba
Bibliographic Information
- Other Title
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- 遺伝子組換え大豆および豆腐中の組換え遺伝子の同定
- Detection of Recombinant DNA in Genetically Modified Soybeans and Tohu
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Abstract
We developed a simple and convenient method for detecting recombinant DNAs providing resistance against 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) inhibition by glyphosate herbicide in soybeans, soybean leaves and tofu. The modified method using CTAB buffer efficiently extracted DNAs. Measurement of the extracted DNAs at 260/230 and 260/280 nm indicated that they were pure enough for PCR amplification. Agarose gel electrophoresis of the PCR amplified products resolved a band at 818 bp for the lectin gene and 513 bp for the introduced gene. The results suggested that our method effectively detects recombinant DNAs in soybeans and soybean products.
Journal
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- Japanese Journal of Food Chemistry and Safety
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Japanese Journal of Food Chemistry and Safety 7 (2), 112-116, 2000
Japanese Society of Food Chemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390001205195352576
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- NII Article ID
- 110007367210
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- NII Book ID
- AA11666400
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- ISSN
- 21896445
- 13412094
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- NDL BIB ID
- 5565665
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- CiNii Articles
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- Abstract License Flag
- Disallowed