Molecular Cloning and Expression of cDNAs Encoding Testis-Specific and Non-Specific ATPase Inhibitor-Like Proteins in Bombyx mori

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  • Ogura Ichiro
    Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
  • Kusakabe Takahiro
    Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
  • Kawaguchi Yutaka
    Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
  • Maeda Takuji
    Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
  • Koga Katsumi
    Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences

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  • Molecular Cloning and Expression of cDNAs Encoding Testis-Specific and Non-Specific ATPase Inhibitor-Like Proteins in <i>Bombyx mori</i>

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A cDNA clone encoding an ATPase inhibitor-like protein was found in a Bombyx mori EST (expressed sequence tag) database and designated as BmAl-a. Also a novel cDNA clone encoding a different ATPase inhibitor-like protein was isolated from a testis library of B. mori after mRNA subtraction, and named BmAl-b. Both BmAl-a and BmAl-b cDNA clones were determined for their nucleotide sequences; the deduced amino acid sequences showed that the relevant proteins were composed of 127 and 107 amino acid residues, respectively. The BmAl-a and BmAl-b proteins have the highest homologies of 41% and 37%, respectively, with the Caenorhabditis elegance ATPase inhibitor-like protein CelF, among the animal homologs so far reported. Expression analysis by reverse transcription polymerase chain reaction demonstrated that the BmAl-a mRNA was transcribed in all tissues examined, while the BmAl-b mRNA was expressed exclusively in the testis. A computational analysis of amino acid residues by a method available from a web-server, suggested that BmAl-a is located in the mitochondria, whereas BmAl-b is allocated in organelles other than the mitochondria.

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