A Possible Procedure to Prepare Mono Fatty Acid-Enriched Plasma Membrane in Chicken Hepatocytes
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- TAKAHASHI Toshihiro
- Department of Animal Science, Faculty of Agriculture, Tohoku University
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- SATO Kan
- Department of Animal Science, Faculty of Agriculture, Tohoku University
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- AKIBA Yukio
- Department of Animal Science, Faculty of Agriculture, Tohoku University
書誌事項
- タイトル別名
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- Possible Procedure to Prepare Mono Fatty Acid-Enriched Plasma Membrane in Chicken Hepatocytes
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説明
In a viewpoint to explore an experimental model to study the regulatory mechanism of hepatic lipogenesis by n-3 fatty acids on hepatic lipogenesis, three experiments were undertaken to prepare mono fatty acid-enriched plasma membrane phospholipid in chicken hepatocytes. Incubation with 500μM α-linolenic acid for 24h in the primary culture of chicken hepatocytes significantly increased α-linolenic acid content and decreased oleic acid content in the plasma membrane phospholipid. In a same fashion, addition of linoleic acid or oleic acid to the media resulted in the higher proportion of the corresponding fatty acid in the plasma membrane. In the subsequent incubation in a fatty acid-free media of α-linolenic acid-enriched cells, α-linolenic acid content decreased gradually with a half life of 24h. Our results suggest that fatty acid supplementation to the culture medium modifies the fatty acid composition of plasma membrane phospholipid of chicken hepatocytes. The present chicken primary culture system may be applicable for investigation of regulatory mechanism of lipid metabolism.
収録刊行物
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- 日本畜産学会報
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日本畜産学会報 70 (3), 118-123, 1999
公益社団法人 日本畜産学会
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詳細情報 詳細情報について
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- CRID
- 1390001205196614400
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- NII論文ID
- 130000742906
- 10020393265
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- NII書誌ID
- AN00195188
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- ISSN
- 18808255
- 00215309
- 1346907X
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- NDL書誌ID
- 4739718
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 使用不可