Hapten Design and Antibody Preparation on Immunoassay toward the Insecticide Oxamyl

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  • MIYAKE Shiro
    Iatron Laboratory, Environmental Immuno-chemical Technology Co., Ltd.
  • MORIMUNE Kosuke
    Naruto Research Laboratories, Environmental Immuno-chemical Technology Co., Ltd.
  • YAMAGUCHI Yuki
    Iatron Laboratory, Environmental Immuno-chemical Technology Co., Ltd.
  • OHDE Katsuya
    Iatron Laboratory, Environmental Immuno-chemical Technology Co., Ltd.
  • KAWATA Mitsuyasu
    Naruto Research Laboratories, Environmental Immuno-chemical Technology Co., Ltd.
  • TAKEWAKI Shunichi
    Iatron Laboratory, Environmental Immuno-chemical Technology Co., Ltd.
  • YUASA Yohjiro
    Environmental Immuno-chemical Technology Co., Ltd.

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  • 殺虫剤オキサミルの免疫化学測定法のためのハプテンデザインと抗体調製

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To detect small amount of oxamyl, indirect competitive enzyme-linked immunosorbent assays. (indirect C-ELISAs) were established. Four haptens for immunogen and three haptens for coating antigen on indirect C-ELISA were synthesized and their polyclonal (PoAbs) and monoclonal antibodies (MoAbs) were prepared. Hapten N, N-dimethyl-2-(5-carboxypentyl) carbamoyloxyimino-2-(methylthio) acetamide was the most effective to raise antibodies toward oxamyl out of haptens for immunogen. MoAbs prepared with this hapten were more highly reactive than PoAbs. In particular, MoAb OXM 6-8 showed the highest reactivity. When N, N-dimethyl-2-(4-carboxycyclohexyl) carbamoyloxyimino-2-(methylthio) acetamide was used for coating antigen on indirect C-ELISA with OXM 6-8, the highest sensitivity was observed and the concentration for 50% inhibition value showed 3.6ng/ml. Methanol extract of potato showed no effect on the reactivity.

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