殺虫剤オキサミルの免疫化学測定法のためのハプテンデザインと抗体調製

DOI NDLデジタルコレクション 日本農学文献記事索引 Web Site Web Site 被引用文献4件 参考文献17件
  • 三宅 司郎
    Iatron Laboratory, Environmental Immuno-chemical Technology Co., Ltd.
  • 森宗 孝介
    Naruto Research Laboratories, Environmental Immuno-chemical Technology Co., Ltd.
  • 山口 優樹
    Iatron Laboratory, Environmental Immuno-chemical Technology Co., Ltd.
  • 大出 勝也
    Iatron Laboratory, Environmental Immuno-chemical Technology Co., Ltd.
  • 川田 充康
    Naruto Research Laboratories, Environmental Immuno-chemical Technology Co., Ltd.
  • 竹脇 俊一
    Iatron Laboratory, Environmental Immuno-chemical Technology Co., Ltd.
  • 湯浅 洋二郎
    Environmental Immuno-chemical Technology Co., Ltd.

書誌事項

タイトル別名
  • Hapten Design and Antibody Preparation on Immunoassay toward the Insecticide Oxamyl

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説明

To detect small amount of oxamyl, indirect competitive enzyme-linked immunosorbent assays. (indirect C-ELISAs) were established. Four haptens for immunogen and three haptens for coating antigen on indirect C-ELISA were synthesized and their polyclonal (PoAbs) and monoclonal antibodies (MoAbs) were prepared. Hapten N, N-dimethyl-2-(5-carboxypentyl) carbamoyloxyimino-2-(methylthio) acetamide was the most effective to raise antibodies toward oxamyl out of haptens for immunogen. MoAbs prepared with this hapten were more highly reactive than PoAbs. In particular, MoAb OXM 6-8 showed the highest reactivity. When N, N-dimethyl-2-(4-carboxycyclohexyl) carbamoyloxyimino-2-(methylthio) acetamide was used for coating antigen on indirect C-ELISA with OXM 6-8, the highest sensitivity was observed and the concentration for 50% inhibition value showed 3.6ng/ml. Methanol extract of potato showed no effect on the reactivity.

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