In Vitro Propagation of Mature Japanese Apricot (Prunus mume Sieb. et Zucc.)

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  • Yonemitsu Hiroshi
    Department of Materials Science, Wakayama National College of Technology
  • Nishi Kazuma
    Department of Materials Science, Wakayama National College of Technology
  • Sagan Saki
    Department of Materials Science, Wakayama National College of Technology
  • Tong Ly
    Department of Materials Science, Wakayama National College of Technology
  • Matsumura Yasuhiro
    Ecosystem Engineering Course, Advanced Engineering Faculty, Wakayama National College of Technology

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Other Title
  • ウメ（Prunus mume Sieb. et Zucc.）の試験管内クローン増殖
  • 繁殖・育苗 ウメ(Prunus mume Sieb. et Zucc.)の試験管内クローン増殖
  • ハンショク イクビョウ ウメ Prunus mume Sieb et Zucc ノ シケンカン ナイ クローン ゾウショク

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Description

Winter buds taken from mature trees of Japanese Apricot (Prunus mume Sieb. et Zucc.) were successfully established in culture on woody plant (WP) agar medium supplemented with 4.4 μM benzyladenine (BA) and 3% glucose. The efficiencies of development of the bud into normal shoots are 50 to 100%. The efficiencies were improved by treating the buds with antioxidants, ascorbic acid, reduced glutathione, ferulic acid, and caffeic acid. Shoot proliferation in subcultures was also best in WP agar medium containing 4.4 mM BA and 3% glucose. Root formation was induced when shoots were transferred into WP agar medium containing 0.54 μM naphthaleneacetic acid. Rooted plantlets were incubated on autoclaved vermiculite containing fertilizer, and growing plantlets were then transferred to soil and successfully acclimated.<br>

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