Establishment of Reporter Yeasts for Guinea Pig and Syrian Hamster Aryl Hydrocarbon Receptor Ligand Activity
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- Kawanishi Masanobu
- Environmental Genetics Laboratory, Frontier Science Innovation Center, Osaka Prefecture University
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- Sakamoto Michiyo
- Environmental Genetics Laboratory, Frontier Science Innovation Center, Osaka Prefecture University
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- Shimohara Chiaki
- Environmental Genetics Laboratory, Frontier Science Innovation Center, Osaka Prefecture University
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- Yagi Takashi
- Environmental Genetics Laboratory, Frontier Science Innovation Center, Osaka Prefecture University
書誌事項
- 公開日
- 2006
- 資源種別
- journal article
- DOI
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- 10.3123/jemsge.28.167
- 公開者
- 日本環境変異原ゲノム学会
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説明
Polymorphism of the aryl hydrocarbon receptor (AhR) presumably induces genetic difference in the susceptibility of animals to aryl hydrocarbons. The activation of intracellular signaling following AhR binding to aryl hydrocarbons is highly correlated with the toxicity and carcinogenicity of these chemicals. Here we developed two reporter yeasts coexpressing AhR and AhR nuclear translocator (Arnt) proteins of a guinea pig and a Syrian hamster, known as the most sensitive and most resistant laboratory rodents to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), respectively. We previously constructed reporter yeasts expressing human and mouse AhR/Arnt. We conducted reporter assays to measure ligand activities of TCDD, 3-methylcholanthrene, β-naphthoflavone and indirubin in these yeasts. Ligand treatment induced a dose-dependent increase in β-galactosidase activity from a reporter plasmid in all 4 yeast strains. The assays showed that yeast expressing guinea pig AhR/Arnt is most sensitive and yeast expressing Syrian hamster AhR/Arnt is most insensitive to these ligands. The yeasts expressing human and mouse AhR/Arnt were in-between. These different ligand activities reflect the species specificity of AhR/Arnt, and may be related to the susceptibility of rodents to aryl hydrocarbons.<br>
収録刊行物
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- Genes and Environment
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Genes and Environment 28 (4), 167-172, 2006
日本環境変異原ゲノム学会
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詳細情報 詳細情報について
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- CRID
- 1390001205257209856
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- NII論文ID
- 130004481146
- 110005717027
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- NII書誌ID
- AA1212552X
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- ISSN
- 18807062
- 18807046
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- NDL書誌ID
- 8684370
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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