DNA Polymerase ^|^lambda; Promotes Mutagenesis Induced by 8-Oxo-7,8-dihydroguanine (8-hydroxyguanine) Paired with Adenine
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- Kamiya Hiroyuki
- Graduate School of Science and Engineering, Ehime University
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- Kurokawa Masahiro
- Graduate School of Science and Engineering, Ehime University
書誌事項
- タイトル別名
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- DNA Polymerase λ Promotes Mutagenesis Induced by 8-Oxo-7,8-dihydroguanine (8-hydroxyguanine) Paired with Adenine
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説明
DNA polymerase λ preferentially inserts dCTP opposite 8-oxo-7,8-dihydroguanine (GO, 8-hydroxyguanine) in vitro, and this function is considered to be important after A base removal from GO:A pairs by the MUTYH DNA glycosylase. However, dCTP incorporation would promote A:T→C:G transversion mutations induced by 8-oxo-7,8-dihydro-dGTP (dGOTP), the mutation triggered by the incorporation of dGOTP opposite A in the template DNA. In this study, double-stranded plasmid DNA containing the GO:A pair, an intermediate in the dGOTP mutagenesis pathway, was transfected into human cells in which DNA polymerase λ was knocked down. The knockdown of DNA polymerase λ significantly reduced the frequency of A:T→C:G transversion mutations induced by the GO:A pair although the knockdown effect was small. These results suggested that DNA polymerase λ is involved in the mutagenesis processes of GO, generated by dGOTP, in the nucleotide pool.<br>
収録刊行物
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- Genes and Environment
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Genes and Environment 35 (4), 105-109, 2013
日本環境変異原学会
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詳細情報 詳細情報について
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- CRID
- 1390001205257244032
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- NII論文ID
- 10031196752
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- NII書誌ID
- AA1212552X
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- COI
- 1:CAS:528:DC%2BC3sXhvVyhurvJ
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- ISSN
- 18807062
- 18807046
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- NDL書誌ID
- 025004111
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- KAKEN
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- 使用不可