気液透過性を付与したTASCLによるhiPS胚様体の高効率生産

DOI

書誌事項

タイトル別名
  • Efficient Formation of Embryonic Bodies by using TASCL with Enhanced Gas-liquid Permeability

抄録

This paper reports on mass production of embryonic bodies (EBs) of human iPS cells by using Tapered Stencil for Cluster Culture device combined with gas/liquid permeable membrane. The device has an array of tapered square microwells, 500μm on a side at the top. The top of the wall separating microwells forms knife-edge so that each cell falls into any one of the microwells. The surface is modified with hydrophilic polymer to prevent cell adhesion. Thus, the cells seeded on the device aggregate at the bottom sealed with porous membrane, and thus, the cells self-assemble into EBs in each microwell. Compared to the previous device with non-permeable bottom, viability of EB were improved by >200% in 6day culture.

収録刊行物

  • 生体医工学

    生体医工学 53 (Supplement), S236_01-S236_01, 2015

    公益社団法人 日本生体医工学会

詳細情報 詳細情報について

  • CRID
    1390001205268204800
  • NII論文ID
    130005163942
  • DOI
    10.11239/jsmbe.53.s236_01
  • ISSN
    18814379
    1347443X
  • 本文言語コード
    ja
  • データソース種別
    • JaLC
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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