Application of comb (chicken) hydrolyzates containing a lot of low-molecular weight hyaluronic acid to the culture of Lentinula edodes and Flammulina populicola

  • TERASHITA Takao
    Laboratory of Food Microbiological Science and Biotechnology, Faculty of Agriculture, Kinki University
  • SHIRASAKA Norifumi
    Laboratory of Food Microbiological Science and Biotechnology, Faculty of Agriculture, Kinki University
  • KUSUDA Mizuho
    Laboratory of Biocycle Engineering, Guraduate School of Life and Environmental Science, Osaka Prefecture University
  • WAKAYAMA Sachio
    Will・Search Co Ltd.

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Other Title
  • シイタケおよびFlammulina populicola培養への低分子ヒアルロン酸を主成分とするニワトリ鶏冠分解物の応用
  • シイタケ オヨビ Flammulina populicola バイヨウ エ ノ テイブンシ ヒアルロンサン オ シュセイブン ト スル ニワトリ ケイカン ブンカイブツ ノ オウヨウ

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The effects of the addition of hydrolyzates containing a lot of low-molecular weight hyaruronic acid (LMW-HA) from comb (chicken) to the culture medium was tested on three commercial strains of Lentinula edodes and Flammulina populicola NBRC 7777 for vegetative mycelial growth and fruit-body yield. The dry weights of mycelia from L. edodes were increased by about 1.75 times (Mori 465 strain), 1.44 times (Meiji Strain) and 1.67 times (Kinko 324 strain) with the addition of comb hydrolyzates (0.125%), respectively. Also, mycelial dry weights and fruit-body yields of F. populicola NBRC 7777 were increased by about 1.70 times and 1.75 times that of the control, respectively. To elucidate the taking of LMW-HA from comb hydrolyzates in cell, N-acetyl-D-glucosamine and glycosaminoglycan contents in mycelia and fruit-bodies of F. populicola were analyzed. N-Acetyl-D-glucosamine contents were increased by about 2.85 times (vegetative mycelia) and 2.69 times (fruit-bodies) with the addition of LMW-HA from comb hydrolyzates in the medium. On the other hand, the concentration of glucosaminoglycan in fruit-bodies was from 0.52% (69.30μg/Petri dish) to 0.35% (469.0μg/Petri dish) even though glucosaminoglycan was not detected in the control culture (without the addition of comb hydrolyzate) when the hydrolyzate (0.05-0.5%) containing LMW-HA was added to the culture medium.

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