Analysis of photosynthetic electron transport in Arabidopsis thaliana under iron deficiency

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  • 鉄欠乏条件におけるシロイヌナズナの光合成電子伝達の解析

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Abstract

Iron is an essential nutrient for plants, and intensive studies have focused on plant responses to iron deficiency. In the field, iron availability often limits the productivity of crops, particularly in alkaline calcareous soil with insoluble iron. Iron is essential for chloroplasts, as a cofactor of many proteins involved in electron transport and reactive oxygen species (ROS) scavenging. Because chloroplasts are the major site of ROS generation, it is important for plants to maintain proper chloroplast function under iron deficiency to avoid photodamage. To study the primary damage to the photosynthetic apparatus by iron deficiency in Arabidopsis thaliana, we took advantage of chlorophyll fluorescence analysis, a nondestructive method of monitoring photosynthetic electron transport in leaves. Chlorosis was observed, and photosystem II (PSII) was severely photodamaged in seedlings grown under severe iron-deficient conditions. In contrast, mild iron deficiency decreased the non-photochemical quenching of chlorophyll fluorescence, which represents the dissipation of absorbed light energy from PSII prior to PSII photodamage. Although transferring seedlings from iron-supplemented to iron-deficient conditions is often used to study plant responses to iron deficiency, photosynthetic electron transport was disturbed before visible symptoms of iron deficiency were observed.

Journal

  • Journal of Crop Research

    Journal of Crop Research 59 (0), 11-15, 2014

    The Society of Crop Science and Breeding in Kinki, Japan

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