Effects of CASF (Ca<SUP>2+</SUP>-activated sarcoplasmic factor) Treatment on Myofibrils Prepared from Rabbit Skeletal Muscles during Storage

  • SUZUKI Atsushi
    Laboratory of Biochemistry and Technology of Animal Products, Faculty of Agriculture, University of Niigata
  • NONAMI Yoshinobu
    Laboratory of Biochemistry and Technology of Animal Products, Faculty of Agriculture, University of Niigata

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Other Title
  • 貯蔵中の家兎筋肉から調製した筋原繊維におよぼすCASF (Ca<SUP>2+</SUP>-activated sarcoplasmic factor) 処理の影響
  • チョゾウチュウ ノ カト キンニク カラ チョウセイシタ キンゲン センイ ニ

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Abstract

Effects of CASF (Ca2+-activated sarcoplasmic factor) treatment on myofibrils prepared from rabbit skeletal muscles during storage were investigated. In this report, myofibrils prepared from at death and 7 days stored muscles were named 0-FM and 7-MF, respectively.<BR>The amount of products released from 7-MF by CASF was less than that from 0-MF, and this observation indicates that the amount of substrate available for CASF in 7-MF is less than that in 0-MF. Acrylamide gel electrophoresis showed that the amount of α-actinin, one of main constituents of Z-line, released from 7-MF was less than that from 0-MF. CASF treatment on 0-MF for 30min caused the increase of Mg-ATPase activity, and CASF treatment on 7-MF for same period caused the decrease of Mg-ATPase activity.<BR>The obtained results in this report suggest an important role of CASF on the changes in myofibrils caused by postmortem storage of the muscle.

Journal

  • Eiyo To Shokuryo

    Eiyo To Shokuryo 30 (6), 371-375, 1977

    JAPAN SOCIETY OF NUTRITION AND FOOD SCIENCE

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