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- TSUTSUMI Nobuhiko
- Laboratory of Radiation Genetics and Chemical Mutagenesis, Faculty of Agriculture, University of Tokyo
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- KANAYAMA Keiko
- Laboratory of Radiation Genetics and Chemical Mutagenesis, Faculty of Agriculture, University of Tokyo
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- TANO Shigemitsu
- Laboratory of Radiation Genetics and Chemical Mutagenesis, Faculty of Agriculture, University of Tokyo
書誌事項
- タイトル別名
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- Suppression of .ALPHA.-amylase gene expression by antisense oligodeoxynucleotide in barley cultured aleurone layers.
- アンチセンス オリゴヌクレオチド ニ ヨル オオムギ アリューロンソウ ニ オケ
この論文をさがす
説明
Antisense oligodeoxynucleotides (ODNs) have been applied to regulate gene expression using cell-free media or animal cells. Here we demonstrate the specific inhibition of barley α-amylase gene expression by synthetic antisense ODNs. In a cell free system using wheat-germ extracts, 5 μM of a 20-mer antisense ODN prevented the synthesis of the polypeptide corresponding to the predetermined length of α-amylase translated in vitro, whereas there was no effect on other protein synthesis. Furthermore, in cultured aleurone cells, a-amylase activity was efficiently decreased by addition of ODNs. At the concentrations higher than 5 μM, antisense ODN inhibited α-amylase gene expression almost completely. These results imply that ODN could transport into the cultured aleurone cells crossing the cell membrane, and regulate specific gene expression. This simple model system could be applicable not only for the analysis of the α-amylase multigene family in barley but also for studying functions of cryptic genes in higher plant.<br>
収録刊行物
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- 遺伝学雑誌
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遺伝学雑誌 67 (2), 147-154, 1992
日本遺伝学会
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詳細情報 詳細情報について
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- CRID
- 1390001205443731328
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- NII論文ID
- 10006699135
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- NII書誌ID
- AA00690924
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- ISSN
- 18805787
- 0021504X
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- NDL書誌ID
- 3773793
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- PubMed
- 1524833
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可