ANTIBODY FORMATION FOR MALIGNANT TUMOR

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  • Antibody formation for malignant tumor-1-Antitumor activity of the regional lymph node protein
  • I. ANTITUMOR ACTIVITY OF THE REGIONAL LYMPH NODE PROTEIN

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Abstract

Lymphoid cells in regional lymph nodes of mice bearing Ehrlich ascites cell tumor have some cytocidal action on cultured Ehrlich ascites carcinoma cells. The regional lymph node protein was fractionated on Sephadex G-200 column and on DEAE-Sephadex A-50 column. The antitumor activity was determined by cell culture test. The antitumor activity was mainly present in the fraction with β-mobility from DEAE-Sephadex A-50 column chromatography. The sedimentation coefficient of this protein was 6.5S. This protein was soluble and permeable through a cell-impermeable diffusion chamber. The minimum protein concentration required for suppression of tumor cell growth was about 300μg for 104 cultured JTC-11 cells in 10ml culture vessel. In electrophoresis, this fraction moved mainly to β-position, and contained a small amount of α-band. The antibody in regional lymph node was loaded mainly on β- and α-mobility protein.

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