Metabolic changes in elicitor-treated <I>Lotus japonicus</I> suspension-cultured cells

DOI
  • Nishida Hiroshi
    Research Association for Biotechnology The NEDO Team of Applied Plant Genomics, Kazusa DNA Res. Inst.
  • Sakurai Nozomu
    The NEDO Team of Applied Plant Genomics, Kazusa DNA Res. Inst.
  • Suzuki Hideyuki
    The NEDO Team of Applied Plant Genomics, Kazusa DNA Res. Inst.
  • Shibata Daisuke
    The NEDO Team of Applied Plant Genomics, Kazusa DNA Res. Inst.

Bibliographic Information

Other Title
  • ミヤコグサ培養細胞における代謝産物解析

Description

Total view of secondary metabolism would be explored best in model plants in which genome organization has been clarified and genetic approach. It has a limit in the secondary metabolism research, as the number of metabolites found in Arabidopsis is lower than those of other plants. Therefore, other model plants could be useful. The legume model Lotus japonicus could be one of the candidates as about 30% of the whole genes show no significant homology with the Arabidopsis genes. We have used suspension-cultured cells of L. japonicus for functional analyses of metabolism-related genes. Our previous study showed that yeast extracts induced several isoflavonoids and expression of related genes. Here we analyzed metabolites of elicitor-treated cultured cells were initiated via callus from hypocotyls of L. japonicus Gifu B129 and hypocotyls or cotyledon of L. japonicus Miyakojima MG20 using LC-MS. We observed different responses in suspension-cultured cells line.

Journal

Details 詳細情報について

  • CRID
    1390001205630957184
  • NII Article ID
    130006991920
  • DOI
    10.14841/jspp.2006.0.887.0
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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