Variation in DNA damage induced by monochromatic soft X-rays in the energy region of oxygen K-edge

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  • SUGAYA Yuki
    Ibaraki University Advanced Science Research Center, Japan Atomic Energy Agency
  • SHIINA Takuya
    Ibaraki University Advanced Science Research Center, Japan Atomic Energy Agency
  • SHIRAISHI Iyo
    Ibaraki University Advanced Science Research Center, Japan Atomic Energy Agency
  • FUJII Kentaro
    Advanced Science Research Center, Japan Atomic Energy Agency
  • YOKOYA Akinari
    Advanced Science Research Center, Japan Atomic Energy Agency

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Other Title
  • 酸素K殼イオン化エネルギー領域の軟X線照射によるDNA損傷の収率変化

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Abstract

We have investigated the site selective damage induction in plasmid DNA by tuning soft X-ray energy using synchrotron radiation as a light source. We reported that the relative yields of base lesions to single strand breaks are strongly depend on the ionization of nitrogen or oxygen atoms in DNA (Fujii et al., 2009). The yield of base lesions at oxygen K-edge is highly more greater than those at nitrogen K-edge. The specific lesions are thought to be induced by direct ionization of DNA. In this study, we aim to reveal the role of direct ionization at the energy region of oxygen K-edge in the selective DNA damage. We measure the yields of single strand breaks (SSBs), base lesions, and AP sites produced in thin film of pUC18 plasmid DNA by irradiation of several soft X-rays which excite a1s electron to anti-bonding energy states or a vacuum level. The yields of base lesions and AP sites are determined by post-irradiation-treatment of the DNA with enzymatic probes, which excise (Nth and Fpg for pyrimidine and purine base lesion, respectively, and Nfo for AP site) and convert the lesions into detectable SSBs. The obtained data will be compared with our previous data obtained by soft X-ray irradiation around carbon and nitrogen K-edge region.

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