Some Properties and Utilization of Respiratory Deficient Mutants of <I>Zygosaccharomyces rouxii</I> for Brewing Miso

DOI

Bibliographic Information

Other Title
  • 耐塩性呼吸欠損変異株の性質と味噌の試醸
  • Some Properties and Utilization of Respiratory Deficient Mutants of Zygosaccharomyces rouxii for Brewing Miso

Abstract

Three mutants (RMM-71, 72, and 73) of salt-tolerant yeast were obtained by combined treatments of ethidium bromide and high-temperature cultivation. The mutants showed respiratory deficiency caused by the loss of cytochrome a+a3, and exhibited lower specific growth rate compared to the parental strain. RMM-71 and 73 were changed to be sensitive to low temperature, their fermentability at 15 °C was decreased than that of the parental strain, and they stopped growth at 10 °C. The mutants kept high salt-tolerance equal to that of the parental strain, and RMM-71 and 73 were changed to be more sensitive to ethanol. Though the mutant produced ethanol similarly to the parent, viable cells of RMM-71 and 73 declined rapidly in barley miso. Miso fermented by the parental strain swelled at 15 °C within a month, wherease RMM-71 and 73 miso hardly swelled at 15 °C for 2 months. Ethanol-tolerance of RMM-71 and 73 was lowered in miso than the parent. From above results, it was suggested that RMM-71 and 73 were more effective than the parent for miso fermentation.

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Details 詳細情報について

  • CRID
    1390001206102529536
  • NII Article ID
    130004306129
  • DOI
    10.6013/jbrewsocjapan1988.91.355
  • ISSN
    21864012
    09147314
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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