ANALYSIS OF HOST-ORIGINATED LIPIDS ASSOCIATING WITH ^|^ldquo;IN VIVO GROWN TUBERCLE BACILLI^|^rdquo;

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  • ANALYSIS OF HOST-ORIGINATED LIPIDS ASSOCIATING WITH “IN VIVO GROWN TUBERCLE BACILLI”

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1) In order to confirm the preceding observation that “in vivo grown tubercle bacilli” harvested from infected mouse lungs were closely associated with host components, lipid analysis was conducted of this material.<BR>2) The 90% acetone extract contained sphingomyelin as the major phospholipid, the neutral lipids including cholesterol in 20%, and free fatty acids such as palmitic, stearic, oleic and linoleic acids.<BR>3) The 90% acetone-insoluble residue was further extracted with chloro-form-methanol (2: 1, v/v) . From this extract, sphingomyelin and lecithin were demonstrated only in a slight amount, insteads cardiolipin was detected as the major phospholipid. Tuberculostearic acid was present in 25% of the constituent fatty acids of the phospholipid fraction. Cholesterol was still detected, but mostly as the ester.<BR>4) These results, together with those in the preceding paper, suggest that the 90% acetone treatment could remove the major portion of host-originated lipids associating with the bacilli grown in vivo and the chloroform-methanol extracted mostly the components proper to the bacilli.<BR>5) Interesting was an additional observation that the ratio of lecithin to sphingomyelin and the ratio of cholesterol to its ester decreased greatly in the bacillus-associated host lipids, probably as a result of the host-parasite interaction.

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