アボカド (Persea americana) 果実の軟化に関係するβ-ガラクトシダーゼのクローニングと発現

DOI NDLデジタルコレクション 日本農学文献記事索引 Web Site Web Site 被引用文献4件 参考文献32件

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タイトル別名
  • Cloning and Expression of .BETA.-Galactosidase cDNA Related to Softening of Avocado (Persea americana) Fruit.
  • Cloning and expression of β-galactosidase cDNA related to softening of avocado(Persea americana) fruit

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cDNA clone encoding a β-galactosidase, AV-GAL, was isolated from fruit of avocado (Persea americana Mill) in which both nucleotide and deduced amino acid sequences had about 70 % identity with those of other plant β-galactosidases. The calculated molecular mass of the presumptive protein, which consists of 766 amino acids, was 85.8 kDa. It contained a putative active site which contained a consensus sequence, G-G-P-[LIVM](2)-x(2)-Q-x-E-N-E-[FY] (amino acid 182-194), belonging to glycosyl hydrolase family 35 and a putative signal peptide which would be cleaved between cysteine and serine (amino acid 35-36). Southern hybridization revealed that avocado β-galactosidase constitutes a small gene family and the probe generated from the 3′-region of AV-GAL, AV-GAL-C, had a higher specificity. Northern blot analysis showed that the AV-GAL-C probe hybridized to a single 3.3 kb RNA. The steady-state level of β-galactosidase mRNA, which was initially detected in the fruit 4 days after harvest, decreased 3 days later. The accumulation of β-galactosidase mRNA temporally corresponded to fruit softening and detectable ethylene production. Moreover, the increase in mRNA accumulation with fruit ripening coincided with changes in the activity of AV-GAL III which is the β-galactosidase isoform most responsible for avocado fruit softening. These results suggest that AV-GAL might be the code for AV-GAL III.

収録刊行物

  • 園芸学会雑誌

    園芸学会雑誌 71 (1), 48-55, 2002

    一般社団法人　園芸学会

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