Spatiotemporal alterations of autophagy marker LC3 in rat skin fibroblasts during wound healing process

DOI 機関リポジトリ Web Site PubMed 被引用文献1件 参考文献22件 オープンアクセス
  • Asai Emiko
    Department of Plastic and Reconstructive Surgery, Fukushima Medical University
  • Yamamoto Masaya
    Department of Anatomy and Histology, Fukushima Medical University
  • Ueda Kazuki
    Department of Plastic and Reconstructive Surgery, Fukushima Medical University
  • Waguri Satoshi
    Department of Anatomy and Histology, Fukushima Medical University

この論文をさがす

説明

<p>To investigate the possible implications of autophagy, one of the degradation pathways induced by metabolic stress, in the dynamic reconstructive process of wound healing, the appearance and changes of punctate structures for microtubule-associated protein 1 light chain 3 (LC3), an autophagosome marker, were examined in a rat skin wound healing model. Although the ratio of LC3-II/LC3-I in Western blotting was not evidently changed during the wound healing process, LC3-positive dots were clearly observed in fibroblasts and myofibroblasts, and occasionally in macrophages, by immunohistofluorescence microscopy. Some of the LC3-positive dots were colocalized with Atg16L signal, an isolation membrane marker, and electron microscopy revealed the presence of typical autophagosomes in fibroblasts near the margin of the wound. The number of LC3-positive dots per fibroblast increased during the later period of the proliferation phase, and interestingly, it was higher in the margin than the center of the wound. It was also high in the periwound skin area. These results suggest that drastic functional changes in fibroblasts during wound healing process are accompanied by the alteration of the autophagy-lysosomal degradation system.</p>

収録刊行物

被引用文献 (1)*注記

もっと見る

参考文献 (22)*注記

もっと見る

関連プロジェクト

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ