無細胞液体培地における鼠癩菌の増菌

中村 昌弘

doi:10.5025/hansen1930.45.4_203

無細胞液体培地における鼠癩菌の増菌

DOI DOI Web Site Web Site Web Site Web Site ほか1件をすべて表示一部だけ表示参考文献11件

中村昌弘

久留米大学医学部微生物学教室細菌学講座

書誌事項

タイトル別名

Multiplication of Mycobacterium lepraemurium in Cell-free Liquid Medium
ムサイボウエキタイバイチニオケルネズミライキンノゾウキン
無細胞液体培地における鼠癩菌の増菌-11-ND-5培地の開発
ムサイボウエキタイバイチニオケルネズミライキンノゾウキン 11
第11報 ND-5培地の開発
11. Establishment of the ND-5 Medium

この論文をさがす

説明

In order to improve the NC-5 medium, the Dubos medium (pH 7.3) was used as a basal medium, instead of Kirchner medium. The complete medium thus prepared is referred to as ND-5 medium. In this medium, Mycobacterium lepraemurium quickly multiplies in the form of binary fission without an extraordinary elongation. Possible generation times could be calculated by repeated experiments as 1.4-2.6 days. A slightly degenerative changes in the cells during prolonged cultivation was observed by electron microscopy. This medium has some advantages for inhibiting other bacterial contaminations. Serial subcultivation is not tested yet.