Conversion of Intravenously Administered .BETA.-Cryptoxanthin to a .BETA.-Carotene-Like Compound in Mouse Lung
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- TOMITA Yoshifumi
- Laboratory of Animal Nutrition and Biochemistry, Migazaki University
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- SENANAYAKE Gamarallage Vijitha Kumara
- Laboratory of Animal Nutrition and Biochemistry, Migazaki University
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- YOSHIOKA Tomoko
- Laboratory of Animal Nutrition and Biochemistry, Migazaki University
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- NISHINO Hoyoku
- Department of Biochemistry, Kyoto Prefectural University of Medicine
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- YANO Masamichi
- Fruit Tree Research Station, Ministry of Agriculture, Forest and Fishery
Bibliographic Information
- Other Title
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- Intravenously Administered β-Cryptoxanthin to a β-Carotene-Like Compound in Mouse Lung
- Intravenously Administered ベータ Cryptoxanthin to a ベータ Carotene Like Compound in Mouse Lung
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Description
The intravenous administration of β-cryptoxanthin (320μg/mouse) into 4-wk-old ddY mice caused a new peak in addition to the peak of β-cryptoxanthin during highperformance liquid chromatography (HPLC) of the lipid fraction of lung homogenate. Different HPLC conditions revealed that the new peak might be attributed to a β-carotenelike compound. The average retention times for the new peak and authentic all-trans-β-carotene were 14.97 and 15.00min, respectively, in a HPLC system using a YMC-Pack ODS-A column and methanol-based mobile phase, and 27.05 and 26.93min, respectively, in a HPLC system using a Waters Nova Pack C18 column and methanol-based mobile phase. In a HPLC system using a Waters Nova Pack C18 column and acetonitrile-based mobile phase, the retention times were 10.73, 10.48 and 10.70min for the new peak, authentic all-trans-β-carotene, and 9-cis-β-carotene, respectively. Spectrophotometry with a photodiode array detector showed maximum absorption of 447 and 475nm for the new peak, and 450 and 475nm for authentic all-trans-β-carotene. This new peak was not observed in the lung tissue of control mice. These findings indicate the possible conversion of β-cryptoxanthin to a β-carotene like-compound in ddY mice.
Journal
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- Journal of Nutritional Science and Vitaminology
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Journal of Nutritional Science and Vitaminology 48 (6), 443-447, 2002
Center for Academic Publications Japan
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Details 詳細情報について
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- CRID
- 1390001206325186176
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- NII Article ID
- 130001377223
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- NII Book ID
- AA00703822
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- ISSN
- 18817742
- 03014800
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- NDL BIB ID
- 6525231
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- PubMed
- 12775109
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL Search
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- CiNii Articles
- OpenAIRE
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- Abstract License Flag
- Disallowed