Intravenously Administered β-Cryptoxanthin to a β-Carotene-Like Compound in Mouse Lung

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タイトル別名
  • Conversion of Intravenously Administered .BETA.-Cryptoxanthin to a .BETA.-Carotene-Like Compound in Mouse Lung
  • Intravenously Administered ベータ Cryptoxanthin to a ベータ Carotene Like Compound in Mouse Lung

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The intravenous administration of β-cryptoxanthin (320μg/mouse) into 4-wk-old ddY mice caused a new peak in addition to the peak of β-cryptoxanthin during highperformance liquid chromatography (HPLC) of the lipid fraction of lung homogenate. Different HPLC conditions revealed that the new peak might be attributed to a β-carotenelike compound. The average retention times for the new peak and authentic all-trans-β-carotene were 14.97 and 15.00min, respectively, in a HPLC system using a YMC-Pack ODS-A column and methanol-based mobile phase, and 27.05 and 26.93min, respectively, in a HPLC system using a Waters Nova Pack C18 column and methanol-based mobile phase. In a HPLC system using a Waters Nova Pack C18 column and acetonitrile-based mobile phase, the retention times were 10.73, 10.48 and 10.70min for the new peak, authentic all-trans-β-carotene, and 9-cis-β-carotene, respectively. Spectrophotometry with a photodiode array detector showed maximum absorption of 447 and 475nm for the new peak, and 450 and 475nm for authentic all-trans-β-carotene. This new peak was not observed in the lung tissue of control mice. These findings indicate the possible conversion of β-cryptoxanthin to a β-carotene like-compound in ddY mice.

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