Some Characteristics of Cytosol Binding Protein for 1α,25-Dihydroxycholecal-ciferol and 25-Hydroxycholecalciferol in Rat intestinal Mucosa

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  • 志村 二三夫
    Department of Nutrition, School of Health Sciences, Faculty of Medicine, University of Tokyo
  • 森内 幸子
    Department of Nutrition, School of Health Sciences, Faculty of Medicine, University of Tokyo
  • 細谷 憲政
    Department of Nutrition, School of Health Sciences, Faculty of Medicine, University of Tokyo

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タイトル別名
  • Some characteristics of cytosol binding protein for 1.ALPHA.,25-dihydroxycholecalciferol and 25-hydroxycholecalciferol in rat intestinal mucosa.
  • Some Characteristics of Cytosol Binding

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Cytosol binding protein for 1α, 25-(OH)2D3 and 25-OHD3 was demonstrated in rat intestinal mucosa using sucrose density gradient ultracentrifugation analysis, Sephadex G-200 column chromatography and polyacrylamide disc gel electrophoresis. The binding protein has a sedimentation constant of 5-6S, a molecular weight of 100, 000-120, 000 daltons and a mobility in electrophoresis of Rf 0.42. Further analysis of binding behavior by DEAE-cellulose filter assays revealed that the binding protein had a higher affinity for 25-OHD3 than for 1α, 25-(OH)2D3, and that 1α, 25-(OH)2D3 competed with 25-OHD3 for the same binding site on the protein. The apparent dissociation constant for 1α, 25-(OH)2D3 and 25-OHD3 was 9.2×10-9 M and 1.5×10-9 M, respectively. The binding capacity was 3.1 pmoles/mg protein for both 1α, 25-(OH)2D3 and 25-OHD3. The order of binding affinity was 25-OHD3>1α, 25-(OH)2D3>>D3=1α-OHD3

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