書誌事項
- タイトル別名
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- A double-antibody enzyme immunoassay for determination of gonadotropin-releasing hormone in bovine plasma.
- ウシ ニ オケル セイセン シゲキ ホルモン ホウシュツ ホルモン ノ 2 コ
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A double-antibody enzyme immunoassay (EIA) for the determining GnRH in bovine plasma was studied. Antiserum raised against [Glu1]-GnRH-rabbit serum albumin conjugate in a male horse was used. Enzyme-labelled antigens were prepared by coupling synthetic GnRH with horseradish peroxidase using bis-diazotized benzidine. GnRH analogues and fragment of GnRH were tested for cross-reactivity in the EIA system. These results suggested that the C-terminus of GnRH molecule plays more important role in antigen-antibody binding than the N-terminus of it.<BR>Plasma specimens were extracted with acid-methanol. Process standard curve was suitable for plasma assay. The sensitivity of this EIA system was as little as 50 pg/tube of GnRH. After intravenous injection of 200 μg of synthetic GnRH in 2 steers, plasma concentrations of GnRH were determined in this EIA system. The levels abruptly increased just after injection and decreased thereafter.
収録刊行物
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- 家畜繁殖学雑誌
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家畜繁殖学雑誌 34 (4), 243-248, 1988
日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001206332725632
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- NII論文ID
- 130004214181
- 40004157416
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- NII書誌ID
- AN00041843
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- NDL書誌ID
- 3224773
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- ISSN
- 03859932
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可