体外成熟マウス卵子の受精能力について

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  • 湊 芳明
    北里大学獣医畜産学部 家畜改良事業団家畜改良技術センター
  • 豊田 裕
    北里大学獣医畜産学部

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タイトル別名
  • Fertilizing ability of mouse oocytes matured in vitro.
  • タイガイ セイジュク マウス ランシ ノ ジュセイ ノウリョク ニ ツイテ

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Primary oocytes surrounded with cumulus cells were obtained from ovarian follicles of prepuberal mice of JCL: ICR strain (25-27 days old) which had been injected with 5 IU PMSG 46-47h previously. The oocytes were cultured for 12h to induce meiotic maturation and then inseminated in vitro with epididymal spermatozoa.<BR>Fertilization was assessed 11-15h later. When the oocytes were matured and fertilized in the basic medium which is a chemically defined medium for in vitro fertilization of mouse oocytes, 38.4% (109/284) of the oocytes showed the evidence of sperm penetration into the vitellus but only 28.4% of these oocytes were found to have male and female pronucleus. The other penetrated oocytes showed either fertilizing sperm tail(s) only or incompletely enlarged sperm head(s) with chromosomes ar-rested or degenerating at metaphase II.<BR>Addition of fetal calf serum (FCS, 1-20%) to the basic medium resulted in an increase in the number of oocytes fertilized (82.1-92.7%) and of the fertilized oocytes with male and female pro-nucleus (59.8-89.5%). Non-dialysable fraction of FCS (5-20%) was effective in increasing the fertiliza-tion rates (53.8-69.0%), while the ultrafiltrated fraction of FCS (5%, molecular weight<10, 000) was effective in increasing the proportion of fertilized oocytes that had male and female pronucleus (72.0%).

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