書誌事項
- タイトル別名
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- Cultivation in vitro of bovine embryos in a medium supplemented with bovine serum collected 7-8 days after superovulation.
- ハツジョウゴ 7 8ニチメ ニ サイシュシタ キョウラン ウシ ケッセイ カバ
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Cultivation in vitlo of bovine embryos in a medium supplemented with bovine serum which was collected from 23 superovulated cows 7 to 8 days after estrus.<BR>Bovine embryos at the stages of morula and blastocyst were cultured at 38 C for 72 h in a stoppered test tube containing 1 ml medium supplemented with 20% bovine serum.<BR>When embryos were cultured in a modified PBS with serum collected on day 7-8 from cows which produced good embryos, 49/57 embryos (86%) were developed to the blastocyst or hatched blastocyst and 83% of the embryos were finally hatched after 72 h of cultivation. On the other hand, when serum, collected from donor cows which produced poor embryos, were supplemented to the medium, none of the 24 embryos was developed.<BR>Effect of bovine serum collected from donor cows on day 7-8 on the developement in vitro of bovine embryo was compared with that of foetus calf serum (FCS). When 20% bovine serum was supplemented to the medium, all 17 frozen-thawed embryos were developed to the blastocyst or hatched blastocyst and the proportion of hatched blastocysts was finally 70%. In contrast, when 20% FCS was supplemented to the medium, 7 of 12 frozenthawed embryos 58% were developed to the blastocystor hatched blastocyst and the final proportion of the hatched blastocysts (22%)was also lower than the rate obtained in the medium including bovine serum.
収録刊行物
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- 家畜繁殖学雑誌
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家畜繁殖学雑誌 31 (1), 1-4, 1985
日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001206333727616
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- NII論文ID
- 130004214069
- 40004157220
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- NII書誌ID
- AN00041843
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- NDL書誌ID
- 3048025
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- ISSN
- 03859932
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可