書誌事項
- タイトル別名
-
- Prenatal diagnosis of sex by the amniotic fluid incattle
- ウシ ノ ヨウスイ ニヨル セイベツ ノ シュッショウゼン シンダン
この論文をさがす
抄録
For the purpose of prenatal diagnosis of sex in cattle, amniotic fluid was asepticaly collected, and the amniotic cells were cultured in vitro in 70 cases; 64 cases were obtained from the pregnant uterus at the time of slaughter, and 6 cases were collected from a pregnant cow.<BR>As the number of amniotic cells per 1ml of amniotic fluid was 5×103-105 (2-6 months of preg-nancy), 10-20ml of amniotic fluid was centrifuged at 1, 000rpm for 5 minutes, and the cell button was dispersed and suspended in a medium of 3ml. The cell cultures were prepared in an incubator at 37C from 8 to 37 days.<BR>Successful cultivation of amniotic cells was obtained by a medium supplemented with bovine fetal serum and N-2-Hydroxyethylpiperadine-N'-2-ethanesulfornic Acid rather than by the other pre-pared mediums.<BR>Amniotic cells from the 3 to 5 months of pregnancy attained the fullest growth in vitro as com-pared with those of the other months.<BR>Twelve cases of successful cultures from the pregnant uterus could be examined chromosomally, and the chromosomal sex and the gonadal sex of the fetus were in good agreement. In 5 out of 6 cases from the pregnant cows, the prenatal chromosomal sex and the gonadal sex of the calf after birth was in agreement, however, in 1 case, a discordance was observed between the prenatal chromo-somal sex and the neonatal gonadal sex.<BR>In addition, a rapid, simple dividing method between amniotic fluid and the allantoic fluid was designed to prevent the false aspiration of allantoic fluid in place of amniotic fluid.
収録刊行物
-
- 家畜繁殖学雑誌
-
家畜繁殖学雑誌 28 (4), 184-188, 1982
日本繁殖生物学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001206333797632
-
- NII論文ID
- 40004157113
- 130004213995
-
- NII書誌ID
- AN00041843
-
- NDL書誌ID
- 2574081
-
- ISSN
- 03859932
-
- 本文言語コード
- ja
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可