書誌事項
- タイトル別名
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- Use of microtitre plate enzyme immunoassay kit for direct determination of progesterone in bovine blood plasma.
抄録
A microtitre plate enzyme immunoassay (EIA) kit for the determination of the concentration of plasma progesterone was evaluated.<BR>The procedure of the EIA kit is very simple and brief; the assay completes in approximately 1.5 hours. Progesterone concentrations in bovine blood plasma can be measured without extraction.<BR>The intra-assay coefficients of variation (CV, N=10) of the absorbances and the concentrations were 4 to 5% and 8 to 16%, respectively. The inter-assay CV (N=8) were 9 to 15%. The inter-assay CV (N=6) of the absorbances of the provided standards in the same batch were 6 to 10%. The detection limit of progesterone was 2.5 pg/well.<BR>The correlation between progesterone concentrations in bovine blood plasma meas-ured by EIA and RIA was quite high (r=0.97, p<0.001).<BR>The results of the present examination suggest that the kit can be used to measure plasma progesterone with sufficient sensitivity and precision, and will be useful for laboratory and veterinary practices.
収録刊行物
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- 家畜繁殖学雑誌
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家畜繁殖学雑誌 33 (3), 134-139, 1987
日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001206333867776
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- NII論文ID
- 130004214150
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- ISSN
- 03859932
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可