Molecular Cloning and Characterization of Porcine Homeodomain Transcription Factor Msx1
-
- ISHIKAWA Akio
- Division of Life Science, Graduate School of Agriculture, Meiji University
-
- KATO Takako
- Institute of Reproduction and Endocrinology, Meiji University
-
- SUSA Takao
- Division of Life Science, Graduate School of Agriculture, Meiji University
-
- SANO Akiko
- Division of Life Science, Graduate School of Agriculture, Meiji University
-
- KATO Yukio
- Division of Life Science, Graduate School of Agriculture, Meiji University Department of Life Science, School of Agriculture, Meiji University Institute of Reproduction and Endocrinology, Meiji University
Search this article
Abstract
We cloned a porcine ortholog of homeodomain transcription factor Msx1 from the porcine pituitary cDNA library. The amino acid sequence of Msx1 shows high conservation among mammalian species. RT-PCR for porcine fetal and postnatal pituitaries showed that Msx1 is already expressed at early fetal day 40, decreases to a low level before birth and then remarkably decreases after birth. On the other hand, Msx1 expression was observed in all pituitary-derived cell line tested, with most in a gonadotrope lineage LβT4. Transfection assay demonstrated that Msx1 markedly repressed the basal Cga and Fshb gene expression, while Lhb expression was affected slightly. Taken together, Msx1 may play a role in repressing gene expression in the fetal and postnatal periods.<br>
Journal
-
- Journal of Reproduction and Development
-
Journal of Reproduction and Development 55 (3), 278-282, 2009
The Society for Reproduction and Development
- Tweet
Details 詳細情報について
-
- CRID
- 1390001206336126976
-
- NII Article ID
- 10024942371
-
- NII Book ID
- AA10936678
-
- ISSN
- 13484400
- 09168818
-
- NDL BIB ID
- 10257849
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
-
- Abstract License Flag
- Disallowed