Birth of normal offspring from mouse eggs activated by a phospholipase Cζ protein lacking three EF-hand domains
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- NAKANISHI Tomoko
- Graduate School of Life and Environmental Sciences, University of Tsukuba Department of Molecular Biology, School of Life Science, Faculty of Medicine, Tottori University
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- ISHIBASHI Naoko
- Graduate School of Life and Environmental Sciences, University of Tsukuba
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- KUBOTA Haruka
- Graduate School of Life and Environmental Sciences, University of Tsukuba
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- INOUE Kimiko
- Institute of Physical and Chemical Research (RIKEN), Bioresource Center
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- OGONUKI Narumi
- Institute of Physical and Chemical Research (RIKEN), Bioresource Center
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- OGURA Atsuo
- Institute of Physical and Chemical Research (RIKEN), Bioresource Center
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- KASHIWABARA Shin-ichi
- Graduate School of Life and Environmental Sciences, University of Tsukuba
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- BABA Tadashi
- Graduate School of Life and Environmental Sciences, University of Tsukuba
書誌事項
- タイトル別名
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- Birth of Normal Offspring from Mouse Eggs Activated by a Phospholipase C.ZETA. Protein Lacking Three EF-hand Domains
- Birth of normal offspring from mouse eggs activated by a phospholipase Cz protein lacking three EF hand domains
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説明
Sperm-specific phospholipase C, PLCζ, is a candidate for the Ca2+ oscillation-inducing factor that is introduced into the ooplasm upon sperm-egg fusion. In addition to the 647-residue full-length PLCζ, s-PLCζ lacking the N-terminal 110 amino acids is known to be present in the mouse testis. In this study, we attempted to obtain full-term offspring from s-PLCζ-activated eggs by round spermatid injection. Metaphase II-arrested eggs injected with a high RNA concentration of s-PLCζ RNA normally developed to blastocysts. When the round spermatid nucleus was injected into telophase II-stage eggs previously activated by s-PLCζ RNA, three live offspring were successfully obtained by transfer of the developed 4-cell embryos to pseudopregnant mice. These three offspring all grew to be normal adults and reproduced healthy second-generation mice.<br>
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 54 (4), 244-249, 2008
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001206336806656
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- NII論文ID
- 10024289241
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- NII書誌ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 9621629
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可