中級脂肪酸Na塩と大豆βーコングリシニンの結合
書誌事項
- タイトル別名
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- Binding of Medium Chain Fatty Acid Anions to Soybean .BETA.-Conglycinin.
抄録
The binding isotherms of aliphatic medium-chain carboxylate anions (octanoate and decanoate) to soy β-conglycinin have been measured by equilibrium dialysis at 30°C in a Tris-HC1 buffer, pH8.0, containing 10mM 2-mercaptoethanol. It has an intrinsic binding constant of 663M-1 for decanoate at 30°C. The number of binding sites on the native β-conglycinin was estimated to be 3. Decanoate binding was independent on the ionic strength and temperature of the incubation medium. Decanoate binding was decreased when reductant was removed from the medium. The native tertiary structure with reduced sulfhydryl group was required maximal binding of decanoate to β-conglycinin. A decrease in decanoate binding occurred when pH of the medium was raised from 8.0 to 10.0, indicating that the presence of positively charged protein sites were required for the binding. The binding affinity was enhanced by an increase in chain length of the ligand, suggesting that the major binding energy is derived from hydrophobic interactions between non-polar tail of fatty acid and hydrophobic regions of protein.
収録刊行物
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- 日本食品工業学会誌
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日本食品工業学会誌 41 (11), 832-839, 1994
社団法人 日本食品科学工学会
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詳細情報 詳細情報について
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- CRID
- 1390001206405953280
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- NII論文ID
- 130003968175
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- ISSN
- 00290394
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可