Alternaria alternateβ‐ガラクトシダーゼの精製とその酵素特性
書誌事項
- タイトル別名
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- Purification and Characteristics of .BETA.-Galactosidase from Alternaria alternate.
説明
Alternaria alternate β-galactosidase was purified and its characteristics were examined. Water extract of crude enzyme obtained from wheat bran culture was purified by salting-out of ammonium sulfate, and by column chromatographies of Sephacryl S-200, DEAETOYOPEARL 650M and Hydroxyapatite. At the final stage, the enzyme was purified to 174-fold activity in comparison with its crude extract and shown unique activity band. Its character were followings; molecular weight was 125000, optimum reaction pH was 4.0-4.5 for ONPG and 5.0-5.5 for lactose, and its stability was kept in the pH range 4.0-8.5. Optimum reaction temperature was about 60°C and heat stability was below 55°C in 1 hour. Km values were indicated respectively 1.56mM for ONPG and 13.9mM for lactose. Inhibitory actions were not observed in the tested reagents.
収録刊行物
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- 日本食品工業学会誌
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日本食品工業学会誌 39 (11), 1023-1029, 1992
社団法人 日本食品科学工学会
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詳細情報 詳細情報について
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- CRID
- 1390001206406814720
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- NII論文ID
- 130003967937
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- ISSN
- 00290394
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可