Ａｌｔｅｒｎａｒｉａ　ａｌｔｅｒｎａｔｅβ‐ガラクトシダーゼの精製とその酵素特性

DOI

書誌事項

タイトル別名
  • Purification and Characteristics of .BETA.-Galactosidase from Alternaria alternate.

抄録

Alternaria alternate β-galactosidase was purified and its characteristics were examined. Water extract of crude enzyme obtained from wheat bran culture was purified by salting-out of ammonium sulfate, and by column chromatographies of Sephacryl S-200, DEAETOYOPEARL 650M and Hydroxyapatite. At the final stage, the enzyme was purified to 174-fold activity in comparison with its crude extract and shown unique activity band. Its character were followings; molecular weight was 125000, optimum reaction pH was 4.0-4.5 for ONPG and 5.0-5.5 for lactose, and its stability was kept in the pH range 4.0-8.5. Optimum reaction temperature was about 60°C and heat stability was below 55°C in 1 hour. Km values were indicated respectively 1.56mM for ONPG and 13.9mM for lactose. Inhibitory actions were not observed in the tested reagents.

収録刊行物

詳細情報 詳細情報について

  • CRID
    1390001206406814720
  • NII論文ID
    130003967937
  • DOI
    10.3136/nskkk1962.39.1023
  • ISSN
    00290394
  • 本文言語コード
    ja
  • データソース種別
    • JaLC
    • Crossref
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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