Comparison of Conventional Antioxidants Assays for Evaluating Potencies of Natural Antioxidants as Food Additives by Collaborative Study

DOI Web Site 16 Citations 31 References
  • Shimamura Tomoko
    Department of Bioresources Science, Faculty of Agriculture, Kochi University
  • Matsuura Ritaro
    Department of Bioresources Science, Faculty of Agriculture, Kochi University
  • Tokuda Takashi
    Department of Bioresources Science, Faculty of Agriculture, Kochi University
  • Sugimoto Naoki
    National Institute of Health Sciences
  • Yamazaki Takeshi
    National Institute of Health Sciences
  • Matsufuji Hiroshi
    Department of Food Science and Technology, College of Bioresource Sciences, Nihon University
  • Matsui Toshiro
    Division of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School Kyusyu University
  • Matsumoto Kiyoshi
    Division of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School Kyusyu University
  • Ukeda Hiroyuki
    Department of Bioresources Science, Faculty of Agriculture, Kochi University

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Other Title
  • 酸化防止剤力価評価のための各種抗酸化活性測定法の共同試験
  • サンカ ボウシザイ リキカ ヒョウカ ノ タメノ カクシュ コウサンカ カッセイ ソクテイホウ ノ キョウドウ シケン
  • Comparison of conventional antioxidant assays for evaluating potencies of natural antioxidants as food additives by collaborative study

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Abstract

In recent years, there has been a need for a new quality criterion for antioxidants based on its antioxidative activity in Japan. In particular, novel official methods of evaluating the antioxidative activities of food additives are required. In order to investigate the possibility of developing official methods, the reproducibilities of three simple conventional antioxidant assays (DPPH assay, ABTS assay, and WST-1 assay) based on spectrophotometric detection were evaluated by collaborative study between three laboratories. DPPH and ABTS assays were performed to evaluate the antioxidant activity (TEAC, Trolox equivalent activity) of all nine antioxidants used in this study, whereas the WST-1 assay was suitable for evaluating the superoxide dismutase (SOD)-equivalent activity of seven antioxidants (excepting D-α-tocopherol and D-δ-tocopherol). The HorRat values from the collaborative study revealed that the DPPH and ABTS assays showed higher reproducibility than the WST-1 assay. It was assumed that the lower reproducibility would be due to the measuring principle, because the WST-1 assay, unlike the DPPH and the ABTS assays, is a competitive enzyme assay. Based on the results, we concluded that the DPPH and ABTS assays would be candidates in validation studies of methods for the purpose of developing an official methodology.

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