書誌事項
- タイトル別名
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- Analysis of Disulfide Bonds in Soybean Proteins using Fluorescence Labeling by Monobromobimane (mBBr)
- ケイコウ シキソ モノブロモバイメイン ヒョウシキ ニ ヨル ダイズ タンパクシツ ジスルフィド ケツゴウ ノ カイセキ
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Disulfides bonds in soybean proteins were estimated by labeling with a fluorescent reagent, monobromobimane (mBBr) and subsequent electrophoresis. Prior to the labeling by mBBr, disulfide bonds in the proteins were chemically reduced by 0.5mM dithiothreitol (DTT) at room temperature. Acidic and basic subunits of glycinin, and Bowman-Birk protease inhibitors were detected as major bands in the soybean proteins. It is found that fluorescence intensity of basic subunit of glycinin correlated significantly to the rupture stress of Tofu using 33 varieties of domestic soybean.
収録刊行物
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- 日本食品科学工学会誌
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日本食品科学工学会誌 51 (8), 406-412, 2004
公益社団法人 日本食品科学工学会
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詳細情報 詳細情報について
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- CRID
- 1390001206410080896
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- NII論文ID
- 10013499166
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- NII書誌ID
- AN10467499
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- ISSN
- 18816681
- 1341027X
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- NDL書誌ID
- 7067155
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
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