Effect of Cysteine Protease Inhibitor on the Setting of Alaska Pollack Surimi Paste.

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  • スケトウダラ塩すり身の坐りに及ぼすシステインプロテアーゼ阻害剤の影響
  • スケトウダラエン スリミ ノ スワリ ニ オヨボス システインプロテアーゼ ソ

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Abstract

Effect of cysteine protease inhibitor on the setting of the Alaska pollack surimi paste was studied in order to clarify the mechanism for the formation of 150kDa protein component in the paste during its setting. The addition of calpain inhibitor I and II to the paste increased the breaking force and the breaking strain of the suwari gel caused at 30°C for 5h and suppressed the formation of the 150kDa protein component. The addition of N-ethylmaleimide or iodoacetamide also suppressed the formation of this component, but remarkably lowered the breaking force and the breaking strain. The EDTA and EGTA scarcely changed the formation. From the results, it was presumed that the 150kDa component was formed by the catalysis of coexisting cysteine protease, and as a result the increment of the breaking force and the breaking strain was suppressed.

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