スケトウダラ肉糊の加温中に生成する150および70kDa成分の同定とその存在状態

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タイトル別名
  • Identification of the 150 and 70 kDa Fragments Generated during the Incubation of Salted Surimi Paste of Walleye Pollack.
  • スケトウダラニクノリ ノ カオン チュウ ニ セイセイ スル 150 オヨビ 70kDa セイブン ノ ドウテイ ト ソノ ソンザイ ジョウタイ

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Incubation of salted surimi paste at 25°C generated the 150 and 70kDa fragments. Two additional bands (bands a and b) migrating between myosin heavy chain monomer (HC) and dimer (HC2) were detected as well. Western blotting analysis of the above bands with anticarp subfragment-1 (s-1) HC antibody and sequencing of the amino terminal region of the 70kDa fragment identified these 150 and 70kDa fragments as heavy meromyosin (HMM)-and light meromyosin (LMM)-like fragments, respectively. The bands a and b are suggested to be the cross-linked product of HC and the 150kDa, and two 150kDa fragments, respectively. Thus, two distinct events, degradation and cross-linking of myosin HC, could occur on the same myosin molecule. These products were proved to be all aggregates losing salt-solubility.

収録刊行物

  • 日本水産学会誌

    日本水産学会誌 66 (5), 869-875, 2000

    公益社団法人 日本水産学会

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