Ultrastructures of OMV infected RTG-2 cells and hepatocytes of chum salmon Oncorhynchus keta.
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- Tanaka Makoto
- Hamanako Branch Shizuoka Fisheries Experimental Station
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- Yoshimizu Mamoru
- Laboratory of Microbiology, Faculty of Fisheries, Hokkaido University
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- Kimura Takahisa
- Laboratory of Microbiology, Faculty of Fisheries, Hokkaido University
Bibliographic Information
- Other Title
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- OMV感染RTG‐2細胞およびサケO.keta肝細胞の超微構造
- OMV感染RTG-2細胞およびサケ O. keta 肝細胞の超微構造
- OMV カンセン RTG-2 サイボウ オヨビ サケ O . keta カン
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Abstract
The ultrastructures of RTG-2 cells and hepatocytes of chum salmon (Oncorhynchus keta) infected with Oncorhynchus mason virus (OMV) were observed by thin sectioning and negative staining. RTG-2 cells were fixed 3 and 7 days after OMV infection (m.o.i.=0.01) and the tissue samples from the liver of moribund 80-day-old, 3-month-old and 4-month-old chum salmon infected with OMV by the immersion method (100 TCID50/ml, 60 min) were prepared for electron microscopy. The infected RTG-2 cells and hepatocytes were prefixed with paraformaldehyde-glutalaldehyde and post fixed with 2% osmic acid.<br>Electron micrographs of both RTG-2 cells and hepatocytes infected with OMV revealed the following activities: nuclei showed the margination and the subsequent aggregation of chromatine; syncytia was found in both RTG-2 cells and hepatocytes; the nuclear membranes became smooth and the virus matured in the nucleus of those cells; the matured virus particles were observed in the vesicle and around the cell membrane; and enveloped virus particles were seen in the focus of the hepatocyte. The size of enveloped virus particles was approximately 200×240nm and the nucleocapsid was 115nm in diameter.
Journal
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- NIPPON SUISAN GAKKAISHI
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NIPPON SUISAN GAKKAISHI 53 (1), 47-55, 1987
The Japanese Society of Fisheries Science
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Keywords
Details 詳細情報について
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- CRID
- 1390001206413161728
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- NII Article ID
- 130001542870
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- NII Book ID
- AN00193422
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- ISSN
- 1349998X
- 00215392
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- HANDLE
- 2115/38616
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- NDL BIB ID
- 3123014
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- Text Lang
- ja
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed