書誌事項
- タイトル別名
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- Construction of the Recombinant Feline Herpesvirus Type 1 Deleted Thymidine Kinase Gene.
- チミジンキナーゼ遺伝子を欠損させた組換え猫ヘルペスウイルス1型の作出〔英文〕
- チミジン キナーゼ イデンシ オ ケッソンサセタ クミカエ ネコ ヘルペスウ
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抄録
We constructed a recombinant virus containing defined deletion within thymidine kinase (TK) gene from feline herpesvirus type 1 (FHV-1) C7301, which was isolated in Japan, by standard rescue procedures with thymidine arabinoside (araT) selection. The araT resistant recombinant virus was designated as C7301dlTK. Southern blot analysis, together with polymerase chain reaction, revealed a deletion with the expected size, based on agarose gel electrophoresis, of the TK gene region in the C7301dlTK. Growth kinetics of the C7301dlTK in CRFK cells was similar to that of the parent C7301 that possesses TK activity. However, plaques produced by the C7301dlTK were significantly smaller than those of the parent virus. This report might be applicable for the development of a new genetically engineered FHV- 1 vaccine.
収録刊行物
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- Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｖｅｔｅｒｉｎａｒｙ Ｍｅｄｉｃａｌ Ｓｃｉｅｎｃｅ
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Ｔｈｅ Ｊｏｕｒｎａｌ ｏｆ Ｖｅｔｅｒｉｎａｒｙ Ｍｅｄｉｃａｌ Ｓｃｉｅｎｃｅ 57 (4), 709-714, 1995
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390001206424511104
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- NII論文ID
- 130003572151
- 110003916353
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- NII書誌ID
- AA10796138
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- COI
- 1:CAS:528:DyaK2MXosF2hu7o%3D
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- ISSN
- 13477439
- 09167250
- http://id.crossref.org/issn/09167250
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- NDL書誌ID
- 3631315
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- PubMed
- 8519903
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可