Canine Amino Acid Transport System Xc⁻ : cDNA Sequence, Distribution and Cystine Transport Activity in Lens Epithelial Cells
-
- MARUO Takuya
- Veterinary Teaching Hospital, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuou-ku, Sagamihara, Kanagawa 252–5201, Japan
-
- KANEMAKI Nobuyuki
- Veterinary Teaching Hospital, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuou-ku, Sagamihara, Kanagawa 252–5201, Japan
-
- ONDA Ken
- Laboratory of Internal Medicine 3, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuou-ku, Sagamihara, Kanagawa 252–5201, Japan
-
- SATO Reiichiro
- Laboratory of Gynecology and Obstetrics, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuou-ku, Sagamihara, Kanagawa 252–5201, Japan
-
- ICHIHARA Nobuteru
- Laboratory of Anatomy 1, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuou-ku, Sagamihara, Kanagawa 252–5201, Japan
-
- OCHIAI Hideharu
- Institute of Biosciences, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuou-ku, Sagamihara, Kanagawa 252–5201, Japan
書誌事項
- タイトル別名
-
- Canine Amino Acid Transport System X<sub>c</sub><sup>–</sup>: cDNA Sequence, Distribution and Cystine Transport Activity in Lens Epithelial Cells
この論文をさがす
抄録
The cystine transport activity of a lens epithelial cell line originated from a canine mature cataract was investigated. The distinct cystine transport activity was observed, which was inhibited to 28% by extracellular 1 mM glutamate. The cDNA sequences of canine cysteine/glutamate exchanger (xCT) and 4F2hc were determined. The predicted amino acid sequences were 527 and 533 amino acid polypeptides, respectively. The amino acid sequences of canine xCT and 4F2hc showed high similarities (>80%) to those of humans. The expression of xCT in lens epithelial cell line was confirmed by western blot analysis. RT-PCR analysis revealed high level expression only in the brain, and it was below the detectable level in other tissues.
収録刊行物
-
- The Journal of Veterinary Medical Science
-
The Journal of Veterinary Medical Science 76 (4), 523-530, 2014
公益社団法人 日本獣医学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390001206430709760
-
- NII論文ID
- 130003382410
-
- NII書誌ID
- AA10796138
-
- COI
- 1:STN:280:DC%2BC2c3ps1Oqtw%3D%3D
-
- ISSN
- 13477439
- 09167250
-
- NDL書誌ID
- 025510230
-
- PubMed
- 24366150
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可