<i>Vibrio parahaemolyticus</i> K<sup>-</sup>株O抗原脂質多糖体の分離と毒性について

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  • Isolation and Toxicity of Lipopolysaccharide of <i>Vibrio parahaemolyticus</i> K minus Strain
  • Vibrio parahaemolyticus K-カブ O コウゲン シシツ タトウタイ ノ ブンリ ト ドクセイ ニ ツイテ

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Lipopolysaccharide (LPS) was extracted from the K minus strain of Vibrio parahaemolyticus with 45% phenol containing 3% NaCl. The resulting crude extract (Fr. I) was further fractionated by centrifugation at 100, 000×g. The precipitated fraction (Fr. II) showed high precipitin activity against O antiserum. Lethality for mice was observed in the Fr. II, but not in the supernatant fraction. The LPS (Fr. II) was hydrolyzed with 1% AcOH at 100° for 1 or 2 hours. The non-dialyzable fraction of the hydrolyzed LPS obtained in a cellophan bag indicated much lower precipitin activity than that of intact LPS.<br>These observations appeared to be the result of liberation of an acid-labile, immunologically active portion from the LPS.<br>Mice injected with the Fr. II died, showing typical endotoxic symptoms and diarrhea.

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