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- Kwon Se Ryun
- Faculty of Fisheries Sciences, Hokkaido University
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- Nishizawa Toyohiko
- Department of Aqualife Medicine, Chonnam National University
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- Takami Ikuo
- Nagasaki Prefectural Institute of Fisheries
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- Yoshimizu Mamoru
- Faculty of Fisheries Sciences, Hokkaido University
書誌事項
- タイトル別名
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- Antibody Detection against Red Sea Bream Iridovirus (RSIV) in Yellowtail Seriola quinqueradiata Using ELISA
この論文をさがす
抄録
FBS components in viral culture fluid interfere with the detection of specific antibody in immunized fish. In the present study, we developed a method to eliminate the interference of FBS using a model of red sea bream iridovirus (RSIV) vaccine. In an experiment using yellowtail antiserum against BSA, which is a major component of FBS in cell culture media, antibodies against BSA were completely absorbed via incubation of antiserum in 50% FBS at 25°C for 1 h. In antibody detection, ELISA with the sera of yellowtail surviving RSIV-challenge, antibodies against RSIV were detected, but those against FBS disappeared via the pre-treatment of the sera. Therefore, antibodies against RSIV are effectively detected using ELISA with the pre-treatment of fish sera.
収録刊行物
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- 魚病研究
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魚病研究 45 (2), 73-76, 2010
日本魚病学会
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詳細情報 詳細情報について
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- CRID
- 1390001206459822336
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- NII論文ID
- 10026514813
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- NII書誌ID
- AN00063165
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- ISSN
- 18817335
- 0388788X
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- HANDLE
- 2115/54120
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- NDL書誌ID
- 10778595
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可