サケ科魚類の伝染性病原体の消毒法に関する研究　１　ＩＰＮウイルスおよびＩＨＮウイルスに対する消毒剤の効力評価法に関する検討

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  • Studies on the method for evaluating virucidal activities of germicides to IPNV and IHNV.
  • IPN ウイルス オヨビ IPN ウイルス ニ タイスル ショウドクザイ ノ
  • サケ科魚類の伝染性病の消毒法に関する研究1

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Optimum methods for evaluating virucidal activities of germicides to infectious pancreatic necrosis virus (IPNV) and infectious hematopoietic necrosis virus (IHNV) were studied.<BR>1. Among the fish cell lines examined CHSE-214 and EPC cells were best suited for examining virucidal activities.<BR>2. Cell-associated virus suspension (CAV) was effectively obtained by freezing and thawing cell-sheet culture just before the appearance of cytopathological effects (CPE).<BR>3. CAV of IPNV did not change in infectivity titer during the stocking period of 8 weeks at either -20°C or -80°C. However, infectivity titer of IHNV in CAV significantly decreased infectivity titer at both -20°C and -80°C.<BR>4. Virucidal activities of IPNV and IHNV did not change during 6 h incubation at temperatures from 10°C to 37°C and from 10°C to 15°C, respectively. Hence, 15°C appears to be a reasonable temperature for routine use in this assay system.<BR>5. Toxicities to IPNV and IHNV of germicides other than halogenic compounds were successfully eliminated by 102-105 dilutions of PBS (-).

収録刊行物

  • 魚病研究

    魚病研究 25 (2), 69-79, 1990

    日本魚病学会

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