Studies on Metabolic Pathway of NAD in Yeast

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  • Part III. Purification of Yeast 5'-Nucleotidase

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Electrophoretically homogeneous preparation of yeast 5'-nucleotidase, one of NAD metabolizing system, was obtained from yeast autolysate by ammonium sulfate fractionation, chromatography, gel filtration and zone electrophoresis. The preparation had strong NAD-splitting activity, namely nucleotide pyrophosphatase activity.<br> Throughout purification steps, the ratio of the two activities were kept constant and they could not be separated even by treatments with EDTA, urea, thioglycol and alkaline buffer. These seem to suggest that both activities of 5'-nucleotidase and nucleotide pyrophosphatase localize on a single protein.

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Details 詳細情報について

  • CRID
    1390001206468173696
  • NII Article ID
    130003522800
  • DOI
    10.1271/bbb1961.31.917
  • COI
    1:CAS:528:DyaF2sXltVGgu7c%3D
  • ISSN
    18811280
    00021369
  • Text Lang
    en
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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