Screening for Drosophila Proteins with Distinct Exprssion Patterns during Development by use of Monoclonal Antibodies
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- KUMAGAI Takeshi
- Graduate Program for Regulation of Biological Signals, Graduate School of Bioagricultural Sciences, Nagoya University
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- YOKOYAMA Hiroaki
- Graduate Program for Regulation of Biological Signals, Graduate School of Bioagricultural Sciences, Nagoya University
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- GOTO Akira
- Graduate Program for Regulation of Biological Signals, Graduate School of Bioagricultural Sciences, Nagoya University
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- HIROSE Junko
- Department of Food and Nutrition, Kyoto Women's University
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- KADOWAKI Tatsuhiko
- Graduate Program for Regulation of Biological Signals, Graduate School of Bioagricultural Sciences, Nagoya University
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- NARITA Hiroshi
- Department of Food and Nutrition, Kyoto Women's University
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- KITAGAWA Yasuo
- Graduate Program for Regulation of Biological Signals, Graduate School of Bioagricultural Sciences, Nagoya University
書誌事項
- タイトル別名
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- Screening for Drosophila Proteins with Distinct Expression Patterns during Development by use of Monoclonal Antibodies.
- Screening for<i>Drosophila</i>Proteins with Distinct Expression Patterns during Development by use of Monoclonal Antibodies
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Kc 167 is a cell line established from Drosophila embryonic hemocytes and has been shown to express many extracellular matrix (ECM) and other proteins important during development. We have screened monoclonal antibodies (mAbs) raised against heparin affinity purified proteins from conditioned medium of Kc 167 cells to identify novel proteins with important roles for development. One mAb recognized a protein expressed with temporary and tissue specific patterns during Drosophila embryogenesis and larval development. This approach is an alternative to screening of Expression Sequence Tag (EST) clones by in situ hybridization to initiate reverse genetics. In addition, a number of mAbs recognizing ECM proteins were also identified. These mAbs will be useful for biochemical and cell biological analyses of Drosophila ECM proteins.<br>
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 64 (1), 24-28, 2000
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206474036480
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- NII論文ID
- 110002679742
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD3cXhtVyitb4%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 5143405
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- PubMed
- 10705444
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可