Role of the DNA Sequence Downstream of the<i>Bacillus subtilis</i><i>hut</i>Promoter in Regulation of the<i>hut</i>Operon

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  • EDA Shima
    Institute of Applied Biochemistry, University of Tsukuba National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, MITI
  • HOSHINO Takayuki
    Institute of Applied Biochemistry, University of Tsukuba
  • ODA Masanao
    National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, MITI

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  • Role of the DNA Sequence Downstream of the Bacillus subtilis hut Promoter in Regulation of the hut Operon.

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  To identify the role of the downstream region of a hut promoter in regulation of the Bacillus subtilis hut operon, three single-base substitutions (+9G→A, +14C→T, and +23T→G) were introduced into the hut operon. Analysis of expression of the hut operon containing each of these three single-base substitutions and the hut-lacZ fusions with the single-base substitutions at position +14 showed that the position at +14 and probably the position at +23 were required for amino acid repression at the hut promoter, while the position at +14 was not required for catabolite repression at the hut promoter. The position at +9 was required for a histidine-dependent increase of activity of the hut promoter. Analysis of expression of the hut-lacZ fusions and the hut operon in the codY mutant indicated that the position at +14 and probably the position at +23 were involved in CodY-mediated amino acid repression at the hut promoter and that CodY was not required for catabolite repression at the hut promoter.<br>

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