Transplantation of a Mammary Stromal Cell Line into a Mammary Fat Pad: Development of the Site-Specific<i>in Vivo</i>Analysis System for Mammary Stromal Cells
-
- NAKATANI Hajime
- Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University
-
- AOKI Naohito
- Department of Life Sciences, Graduate School of Bioresources, Mie University
-
- NADANO Daita
- Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University
-
- MATSUDA Tsukasa
- Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University
書誌事項
- タイトル別名
-
- Transplantation of a Mammary Stromal Cell Line into a Mammary Fat Pad: Development of the Site-Specific in Vivo Analysis System for Mammary Stromal Cells
この論文をさがす
抄録
The interaction between mammary epithelial and stromal tissue is considered to be important in breast tissue development. In this study, we developed a transplantation procedure for the mammary stromal fibroblastic cell line (MSF) to examine its life in vivo. First we established MSF cells which stably expressed lacZ (lacZ/MSF) and had characteristics of mammary stromal cells. The lacZ/MSF cells were then transplanted into a cleared mammary fat pad of syngenic mice with and without mammary primary epithelial organoids. Whole mount X-gal and carmine staining of the transplants revealed that a number of undifferentiated lacZ/MSF cells survived around the mammary epithelial tissue when transplanted with organoids. These results indicate that transplantation of MSF cells into mammary fat pad was accomplished by co-transplantation with primary mammary organoids. Finally, we discuss the application of transplantation procedure for in vivo studies of the mammary stromal tissue development and stromal-epithelial interactions.
収録刊行物
-
- Bioscience, Biotechnology, and Biochemistry
-
Bioscience, Biotechnology, and Biochemistry 75 (3), 550-555, 2011
公益社団法人 日本農芸化学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001206477094272
-
- NII論文ID
- 10028201934
-
- NII書誌ID
- AA10824164
-
- ISSN
- 13476947
- 09168451
-
- NDL書誌ID
- 11051777
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可