Isolation of Protoplast from Aerial Mycelia and Fruit-Body Formation from Regenerated Mycelia in<i>Pleurotus cornucopiae</i>

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  • MIZOGUCHI Masahide
    Department of Biology, Faculty of Science and Engineering, Konan University
  • KITANO Satoshi
    Department of Biology, Faculty of Science and Engineering, Konan University
  • TAKAHASHI Wataru
    Forage Crop Research Institute, Japan Grassland Agriculture and Forage Seed Association
  • SUGAWARA Fuyuki
    Akita Prefecture Forest Technical Center
  • TANAKA Osamu
    Department of Biology, Faculty of Science and Engineering, Konan University

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  • Isolation of Protoplast from Aerial Mycelia and Fruit-Body Formation from Regenerated Mycelia in Pleurotus cornucopiae

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We have developed a simple and efficient method for protoplast isolation from Pleurotus cornucopiae. Protoplasts were isolated from aerial mycelia cultured on potato dextrose agar medium without time-consuming propagation in liquid culture. Protoplast yield was significantly increased by means of a decompressing pretreatment of mycelia in enzyme solution and a subsequent enzyme reaction with vibrational mixing. The isolated protoplasts regenerated mycelia and these mycelia formed fruit-bodies without any morphological abnormalities.

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